RT-PCR Question

Hiranya Roychowdhury hroychow at NMSU.EDU
Thu Oct 17 09:38:29 EST 1996


At 12:35 AM 10/17/96 GMT, Peter J Bartholomew wrote:
>Hello,
>As a minor part of my research project, I isolated total RNA from
>estradiol-treated and control cells followed by RT-PCR.  This was done
>to compare the mRNA levels of a certain gene.  Since the resultant
>bands seemed of equal intensity following agarose gel electrophoresis
>and ethidium bromide staining, I stated that the estradiol treatment
>did not affect the mRNA levels.  In retrospect, I know I should have
>done a northern, but was this conclusion still acceptable?
>Thank you.  
>
>

Who is supervising your research project? If anybody is, shouldn't that
person be the one answering your questions?


Dr. Hiranya Sankar Roychowdhury
Plant Genetic Engineering Lab.
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at nmsu.edu




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