At 04:08 PM 10/16/96 +0200, Manuel G. Claros wrote:
>On 14 Oct 1996, Guido Hooiveld wrote:
>> > I was told an A280/A260 ratio
>> > theoretically limit is 2.0. However, I often find a ratio larger than
2.0; like 2.1 to 2.2. So,
> > is this (theoretically) value of 2.0 correct, if yes, does anybody have
a suggestion why I find
> > values >2; if not, what is this value?
>>You have degradated nucleotides.
No, ...degraded nucleic acids.
>> > multiply A260 with 50 to obtain the concentration (ug/ml), or use 35?
Which value is correct?
>>50 for oligonucleotides and 35 for dsDNA
No!! ...It is the other way around.
>> > Any comments would be appreciated!
>>I hope this helps
>>Manuel G. CLAROS, Ph.D.
>Bioq. & Biologia Molecular
>Campus de Teatinos
Dr. Hiranya Sankar Roychowdhury
Plant Genetic Engineering Lab.
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at nmsu.edu