Lumi-Phos 530; where?
kraev at bc.biol.ethz.ch
Thu Oct 17 09:25:11 EST 1996
In article <dboyd-1410961639550001 at cc-hsc-k-box26.cc.umanitoba.ca>,
dboyd at cc.umanitoba.ca (DAB) wrote:
> I finished my Lumi-Phos 530 from my Photogene kit (GIBCO/BRL Life
> Technologies) but have lots of SA-AP and Final Wash solution left but they
> won't sell me just some Lumi-Phos; they want me to buy a whole new kit
> (typical). Does anybody know whwre I can get some Lumi-Phos compatible
> with the Photogene kit and its Final Wash solution?
You may try to contact Tropix ( FAX 617-275-8581, Tel 617-271-0045), I
should be able to offer you a substitute. I did not try it with
Photogene, since I
make my own biotin and DIG detection solutions, but the "final wash" should be
either TEA-MgCl2 or tris-HCl-MgCl2 and you should be able to use any of
substrates, e.g. CSPD or CDP-Star. Usual disclaimers...
I hope this helps,
Alexander Kraev, PhD
Biochemie III, ETHZ Zurich
e-mail kraev at bc.biol.ethz.ch
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