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Primer extension method of ALF express

Ant Smith ant at molbiol.uct.ac.za
Thu Oct 17 17:08:50 EST 1996


Hi

Can anyone provide me with a protocol for doing primer extensions using 
the ALF express automated DNA sequencer. I have not seen any kits for 
this provided by Pharmacia. 

I have the unlabeled primers so a method using incorparation is what I 
require.

Thanks
Ant Smith
Department of Microbiology
University of Cape Town
South Africa



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