Diane Judd (d.judd at ic.ac.uk) wrote:
:: I would like to know is it possible to lyse fixed cells effectively? And if
: so, how?
:: I am trying to ascertain how fixation (4% formaldehyde) effects the
: fluorescence of a particular protein in cos7 cells and therefore want to
: lyse them after fixation in order to measure fluorescence of the lysate in a
Forgive me if I am wrong but does not formaldehyde fix by cross-linking
proteins, etc. What makes you think the protein will be released?