lysing of fixed cells

Michael Cooley szcooley at chip.ucdavis.edu
Thu Oct 17 06:43:56 EST 1996


Diane Judd (d.judd at ic.ac.uk) wrote:
: 
: I would like to know is it possible to lyse fixed cells effectively? And if
: so, how? 
: 
: I am trying to ascertain how fixation (4% formaldehyde) effects the
: fluorescence of a particular protein in cos7 cells and therefore want to
: lyse them after fixation in order to measure fluorescence of the lysate in a
: fluorimeter.
: 
: 
: Diane
: 

Forgive me if I am wrong but does not formaldehyde fix by cross-linking
proteins, etc. What makes you think the protein will be released?



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