lysing of fixed cells
szcooley at chip.ucdavis.edu
Thu Oct 17 06:43:56 EST 1996
Diane Judd (d.judd at ic.ac.uk) wrote:
: I would like to know is it possible to lyse fixed cells effectively? And if
: so, how?
: I am trying to ascertain how fixation (4% formaldehyde) effects the
: fluorescence of a particular protein in cos7 cells and therefore want to
: lyse them after fixation in order to measure fluorescence of the lysate in a
Forgive me if I am wrong but does not formaldehyde fix by cross-linking
proteins, etc. What makes you think the protein will be released?
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