Below is one response that I received from my inquiry about the use of
myeloma cell lines for the expression of recombinant, non-antibody
proteins. I thank the correspondent for the help.
Gregg Wells
Department of Pathology & Laboratory Medicine
University of Pennsylvania
Philadelphia, PA 19104-6082
USA
email: wellsg at mail.med.upenn.edu
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>In fact, Celltech Therapeutics in the UK has built an entire business
>around their technologies and patents to express heterologous proteins
>from myeloma cells. In particular, they use the NS0 myeloma and a
>glutamine synthetase selection marker.
>Many years ago I shared your expectation that myeloma cells would be
>excellent for expression since they are basically professional
>secretory cells. The Celltech experience has shown that is true. But
>surprisingly, they are not actually better than recombinant CHO cells
>amplified through the dhfr/methotrexate amplification system. In the
>past two years I have had experience with two different recombinant
>CHO lines obtained from two different outside labs that both expressed
>heterologous proteins at more than 40-50 pg/cell/d, or over 0.3-0.5
>grams per liter in a batch culture! Very few myelomas express at this
>level naturally.
>So, yes, myelomas are good hosts, but not necessarily better hosts for
>expression.
