"FABIAN MOEBIUS" <Fabian.Moebius at uibk.ac.at> wrote:
>I need to sublone a 14 kb fragment. Is 10 kb an absolute maximum for the insert size for pBS? Is someone
>aware of a plot between insert size and pBS/pBS-like plasmids cloning effiency?
>Best regards and nice weekend
>Dr. Fabian F. MOEBIUS
>Institut fuer Biochemische Pharmakologie
>Peter Mayr Str. 1
I've currently got 22 kb into pBS and hoping to fit another 8 in. The
yield of DNA has gone right down, but what the hell, I like growing
bugs by the litre.
Institute for Animal Health
clive.tregaskes at bbsrc.ac.uk