pBS max insert size

[Clive Tregaskes]

"FABIAN MOEBIUS" <Fabian.Moebius at uibk.ac.at> wrote:

>Hello,
>I need to sublone a 14 kb fragment. Is 10 kb an absolute maximum for the insert size for pBS? Is someone 
>aware of a plot between insert size and pBS/pBS-like plasmids cloning effiency?
>Best regards and nice weekend
>Fabian



>Dr. Fabian F. MOEBIUS
>Institut fuer Biochemische Pharmakologie
>Peter Mayr Str. 1
>6020 Innsbruck
>AUSTRIA
>TEL ++43-512-507-3155
>FAX ++43-512-588627
I've currently got 22 kb into pBS and hoping to fit another 8 in. The
yield of DNA has gone right down, but what the hell, I like growing
bugs by the litre.

Clive Tregaskes
Institute for Animal Health
Compton
Newbury
UK
clive.tregaskes at bbsrc.ac.uk