DNA onto metal

Mircea Podar podar at utsw.swmed.edu
Fri Oct 18 20:01:39 EST 1996

In article <54627h$h04 at news3.realtime.net>, Lesley Lakey
<LLakey at CarboMedics.com> wrote:

> I am looking for methods of precipitating plasmid DNA onto metals such as 
> gold, platinum, and iridium.  Any suggestions?  I am doing gene therapy 
> research and need a quick release of DNA to the area of interest.
>                                                   Thanks,
>                                                   L. Lakey

Check the article published in Methods in Enzymology, vol 264 (1996),
pages 265-278. That describes a protocol for coating gold microbeads with
plasmid DNA for use in the mitochondrial transformation of yeast with the
"biolistic gun". It works also for tungsten. 


More information about the Methods mailing list