DNA onto metal

Mircea Podar podar at utsw.swmed.edu
Fri Oct 18 20:01:39 EST 1996


In article <54627h$h04 at news3.realtime.net>, Lesley Lakey
<LLakey at CarboMedics.com> wrote:

> I am looking for methods of precipitating plasmid DNA onto metals such as 
> gold, platinum, and iridium.  Any suggestions?  I am doing gene therapy 
> research and need a quick release of DNA to the area of interest.
>                                                  
>                                                   Thanks,
>                                                   L. Lakey



Check the article published in Methods in Enzymology, vol 264 (1996),
pages 265-278. That describes a protocol for coating gold microbeads with
plasmid DNA for use in the mitochondrial transformation of yeast with the
"biolistic gun". It works also for tungsten. 

Mircea



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