Transfection of Drosophila Schneider cells

User please at use.adress.in.signature
Fri Oct 18 14:23:01 EST 1996


Hello !
Is there anyone who has experience in 
calcium-phosphat-coprecipitation of Drosophila Schneider cells S2 ?
The problem is, that I cannot detect any RNA of my transfected DNAs
by Nuclease S1 - Analysis: For example : VAI is a high expressed 
RNA Pol III - Gen, but the RNA is undetectable after RNA-Preparation
by the GIT-method.
Have you got any tips for a solution.

Thanks for your time !

Björn Sandrock
AG Benecke
Ruhr-Uni-Bochum, Germany



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