Pamela Lagali (plagali at gpu2.srv.ualberta.ca) wrote:
: Hello,
:: I am looking for a protocol for stripping (nylon) Southern blots. I have tried
: using a boiling 0.1% SDS solution poured over the blot and allowed to cool to
: room temperature (i.e. the "harsh treatment" for stripping as prescribed by
: "Current Protocols in Molecular Biology"). This method removes most of the
: hybridized probe, but some of it remains in addition to a significant amount of
: background signal. I performed the above procedure twice, and there was no
: appreciable reduction in signal intensity when the blot was re-exposed to film
: for the second time. I am confident that the blot was not allowed to dry out at
: any point (but I can never be sure I guess). Does anyone know of any HARSHER
: stripping procedures which I could try to attempt to remove what remains of the
: hybridized probe?
:: Thanks for any suggestions,
:: Pam
:::: Pamela Lagali
: Department of Cell Biology
: 6-50 Medical Sciences Building
: University of Alberta
: Edmonton, Alberta, Canada
: T6G 0X6
: ph: (403) 492-7407
:
I'm convinced that harsher conditions are ineffective. Please see my post
to you via email and my post on this bullitin board. Thanks, Mike Cooley
