Isolation mtDNA

John Ladasky ladasky at leland.Stanford.EDU
Fri Oct 18 02:39:06 EST 1996


In article <199610170446.LAA02258 at meiosis.eijkman.go.id>,
Djumadi LF <djum at eijkman.go.id> wrote:
>I have a problem How to isolation mtDNA from human tissue various (minus 12
>hours of post-mortem).
>I wolud like to get are methods.
>Thanks 

	If I remember correctly, the alkaline/acid technique used for iso-
lating plasmid DNA from bacteria was originally developed for mitochondrial
DNA!  The mitochondrial genome, like plasmids, is in a closed circular form
and thus resists permanent denaturation in strong base.  You might want to
look up some papers from David Clayton written in the late 60's through the
early 70's... I think.

	Good luck!

-- 
Unique ID : Ladasky, John Joseph Jr.
Title     : BA Biochemistry, U.C. Berkeley, 1989  (Ph.D. perhaps 1998???)
Location  : Stanford University, Dept. of Structural Biology, Fairchild D-105
Keywords  : immunology, music, running, Green



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