Dear sir
In my research, I use PCR for identifying my clone. In my laboratory,
I have some PCR diagnose Kits from some suppliers which add agarose
gel loading solution directly to pcr mixture. When finished PCR cycles,
the PCR mixture were loaded directly to agarose gel for electrophoresis.
I am not sure if the bromophenol blue and sucrose in loading solution
could affect the PCR result. If the bromophenol blue and sucrose do not
affect the PCR result, what is the end concentration of the bromophenol
blue and sucrose in PCR reaction?
Please send the reply directly to me by email.
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Ouyang Hongsheng Department of Biochemistry
Ph.D. Changgchun University of Agriculture
email:ouyhs at public.cc.jl.cn and Animal Sciences
Tel:86-431-7983451 175 Xian Road, Changchun130062
Fax:86-431-7983442 P. R. China
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Thanks.
Ouyang Hongsheng
Ph.D.
