Has anyone else had a problem with analysing putative positive clones from
a lambda gt10 cDNA library?
We routinely screen are positives by restriction digest and PCR (using
forward and reverse lambda gt10 primers) to determine the insert size.
However, one postive (which has been re-probed numerous times with both
cDNA probes) fails to show an insert. We are at a loss to explain this.
Has this happened elsewhere??????
TIA for your comments and observations
iow at aber.ac.uk