lambda gt10 libraries

[EFDG]

Has anyone else had a problem with analysing putative positive clones from 
a lambda gt10 cDNA library?

We routinely screen are positives by restriction digest and PCR (using 
forward and reverse lambda gt10 primers) to determine the insert size.  
However, one postive (which has been re-probed numerous times with both 
cDNA probes) fails to show an insert.  We are at a loss to explain this.

Has this happened elsewhere??????

TIA for your comments and observations

Stuart

iow at aber.ac.uk