lysis problems with bacteriophage cultures

Dr. Duncan Clark duncan at
Sun Oct 20 04:56:35 EST 1996

In article <544rtg$lev at>, Artur da Camara Machado
<iam at> writes
>has anyone an idea why bacterial cultures (KW251) inoculated with lambda-GEM11
>do not lyse after overnight incubation at 37°C.

KW251 is only supE. Does it need supF else it forms a lysogen? A quick
look at Promega's literature show that all their listed permissive hosts
are supF.

My mind's made up. Don't confuse me with the facts!
Duncan Clark

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