Q: polyA tail length vs. mRNA capture

Eric Lader lader at ambion.com
Sun Oct 20 21:36:46 EST 1996


 Steven Sullivan, sullivan at gwis2.circ.gwu.edu writes:
>
>1) anyone know what the *shortest* length of polyA is that gets captured on 
>oligo dT columns or hybridized to oligodT primers (e.g. LTI's oligo dT12-18)?
>
We frequently purify probes with 20 A residues with good efficiency. I
also know for sure tha 20 A's is good enough for dT priming in RT-PCR.


               **my opinions are just that**
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        Eric Lader Ph.D.	Senior R&D Scientist,  
                          Ambion Inc.
Unique tools for Molecular Biology Research

Phone (800) 888-8804       Fax (512) 445-7139
				  (512) 445-6979       lader at ambion.com
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