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antisense expression

Brent bilha001 at gold.tc.umn.edu
Sun Oct 20 19:59:51 EST 1996

      I am just getting into the bussiness of expressing antisense RNA
using retrovirus vectors. Building the construct is a diffecult problem.
The only cloning strategy I have come up with involves PCR to change
restriction sites so that the antisense is expressed. Is there a strategy
I have missed? Any advice will be helpful. 
                          An email response is fine.

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