Factors affecting the effciency of end-labelling by DNA kinase

Karl Fischer tyr-2 at bones.biochem.ualberta.ca
Mon Oct 21 14:32:03 EST 1996


In article <54g99r$tn5 at enyo.uwa.edu.au>, dvoon at cyllene.uwa.edu.au (Dominic
Voon) wrote:

>         I am going to start labelling some PCR products using DNA kinase
> (with alpha-32P dCTP) (etc.)

Alpha labelled?? Deoxycytidine? For a kinase reaction???

This is new to me...I label fragments/oligos with gamma 32P rATP in a PNK
reaction but have never heard of what you are proposing.
Sigh.... ;-)

Karl the hepB guy

-- 
Karl Fischer
tyr-2 at bones.biochem.ualberta.ca





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