why G418 with stable lines?

Joe Miano jmiano at post.its.mcw.edu
Tue Oct 22 00:10:49 EST 1996


Question:

If a transgene is stably integrated in a cell line through selection with
G418, why is continued G418 necessary with clonally-derived cells?  It
seems that once the gene is integrated and cloned, no further G418 would be
necessary, n'est ce pas?
-- 
The Love you take is equal to the Love you make--P. McCartney



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