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why G418 with stable lines?

Joe Miano jmiano at post.its.mcw.edu
Tue Oct 22 00:10:49 EST 1996


If a transgene is stably integrated in a cell line through selection with
G418, why is continued G418 necessary with clonally-derived cells?  It
seems that once the gene is integrated and cloned, no further G418 would be
necessary, n'est ce pas?
The Love you take is equal to the Love you make--P. McCartney

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