Factors affecting the effciency of end-labelling by DNA kinase
dvoon at cyllene.uwa.edu.au
Mon Oct 21 11:44:43 EST 1996
I am going to start labelling some PCR products using DNA kinase
(with alpha-32P dCTP) and it is quite important that I get high
effeciency. I am just wondering if anyone could just give me a quick
run-down on the critical factors that contribute to the effeciency of this
experiment ? such as DNA concentration, dCTP concentration etc. Any
pointer/comment/suggestion/advice will be much appreciated. Please email
me directly at dvoon at cyllene.uwa.edu.au.
many thanks in advance,
Dominic Voon .***. .***. .***. .***.
Department of Biochemistry *| | | * | | | * * | | | * | | | *
University of Western Australia | | * * | | | * * | | | * * | | | *
Nedlands 6907,Perth * | | * * | | | * | | | * * | | | *
Australia '***' '***' '***' '***'
Tel: (609) 380 2303 (w) Fax: (609) 380 1148
386 6036 (h)
Email:dvoon at cyllene.uwa.edu.au http://www.uwa.edu.au/cyllene/dvoon
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