Factors affecting the effciency of end-labelling by DNA kinase

[Dominic Voon]

Hi all,
	I am going to start labelling some PCR products using DNA kinase
(with alpha-32P dCTP) and it is quite important that I get high
effeciency. I am just wondering if anyone could just give me a quick
run-down on the critical factors that contribute to the effeciency of this
experiment ? such as DNA concentration, dCTP concentration etc. Any
pointer/comment/suggestion/advice will be much appreciated. Please email
me directly at dvoon at cyllene.uwa.edu.au.

many thanks in advance,
dominic
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Dominic Voon                    .***.   .***.           .***.   .***.
Department of Biochemistry     *| | | * | | | *       * | | | * | | | *
University of Western Australia | | *   * | | | *   * | | | *   * | | | *
Nedlands 6907,Perth         * | |  *      * | | | * | | | *       * | | | *
Australia                    '***'          '***'   '***'           '***'
Tel: (609) 380 2303 (w)                     Fax: (609) 380 1148
           386 6036 (h)	
Email:dvoon at cyllene.uwa.edu.au           http://www.uwa.edu.au/cyllene/dvoon
Check out the OCF Perth Home Page:
        http://ccmb.microbiol.uwa.edu.au/~dvoon/ocf/ocf-home.html
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