When crosslinking with UV what/where are the covalent bonds made? With which
part of the nucleic acid and nylon?
Also, I have assumed that when you crosslink RNA to a positively charged membrane
by using NaOH you deprotonate the 2'hydroxy, the anion then acts as a nucleophile
and it attacks a positive center in the nylon - would that be accurate?
Thank you to all in advance!