What happens when you crosslink nucleic acid to nylon?

Edmundo Castro ecastro at physci.ucla.edu
Mon Oct 21 16:04:59 EST 1996


When crosslinking with UV what/where are the covalent bonds made?  With which 
part of the nucleic acid and nylon?

Also, I have assumed that when you crosslink RNA to a positively charged membrane 
by using NaOH you deprotonate the 2'hydroxy, the anion then acts as a nucleophile 
and it attacks a positive center in the nylon - would that be accurate?

Thank you to all in advance!

E. Castro-Vargas



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