Nylon membrane stripping: deionized formamide....
ecastro at physci.ucla.edu
Mon Oct 21 16:00:26 EST 1996
I was wondering what people use in order to strip their RNA nylon membranes. I
have used boiling 0.1% SDS or 50% formamide 6XSSPE to strip uncharged and
positively charged membranes before. The second method seems to work better. Has
anyone tried something with wich they are happy with?
Also, I have been using deoinized formamide: is this a requirement when you are just
trying to strip the blot?
Thank you for your time!
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