I was wondering what people use in order to strip their RNA nylon membranes. I
have used boiling 0.1% SDS or 50% formamide 6XSSPE to strip uncharged and
positively charged membranes before. The second method seems to work better. Has
anyone tried something with wich they are happy with?
Also, I have been using deoinized formamide: is this a requirement when you are just
trying to strip the blot?
Thank you for your time!