DNA Sequencing

William Kopaciewicz Willk at seacoast.com
Sat Oct 19 19:48:23 EST 1996


A recent article in Biotechniques 21:453-457 (Sept 1996) reports
the use of heat soaked PCR protocols (HS-PCR) in automated DNA
sequencing reactions to obviate DNA template purification up to 500 bp
with insignificant background.  Is anyone familiar with this
technique and if so, do they agree with the authors that the overall
quality of DNA sequence is considerably improved and that it is becoming
unnecessary to improve the quality of DNA templates (i.e. use DNA
directly from E.Coli without purification)?  Thank you greatly for your
response in advance.



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