Nylon membrane stripping: deionized formamide....

Colin Rasmussen colin at fungus.biochem.ualberta.ca
Mon Oct 21 23:04:52 EST 1996


In article <326BE46A.4183 at physci.ucla.edu>, ecastro at physci.ucla.edu wrote:

> Hello all!
> 
> I was wondering what people use in order to strip their RNA nylon
membranes.  I 
> have used boiling 0.1% SDS or 50% formamide 6XSSPE to strip uncharged and 
> positively charged membranes before.  The second method seems to work
better.  Has 
> anyone tried something with wich they are happy with?
> 
> Also, I have been using deoinized formamide:  is this a requirement when
you are just 
> trying to strip the blot?

I use 0.1xSSC, 0.1% SDS, and boil for 5 minutes or so.  Seems to work very
well for what we have done.

Colin

-- 
Colin Rasmussen
Molecular Mechanisms in Growth Control Group
University of Alberta



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