In article <326BE46A.4183 at physci.ucla.edu>, ecastro at physci.ucla.edu wrote:
> Hello all!
>> I was wondering what people use in order to strip their RNA nylon
membranes. I
> have used boiling 0.1% SDS or 50% formamide 6XSSPE to strip uncharged and
> positively charged membranes before. The second method seems to work
better. Has
> anyone tried something with wich they are happy with?
>> Also, I have been using deoinized formamide: is this a requirement when
you are just
> trying to strip the blot?
I use 0.1xSSC, 0.1% SDS, and boil for 5 minutes or so. Seems to work very
well for what we have done.
Colin
--
Colin Rasmussen
Molecular Mechanisms in Growth Control Group
University of Alberta
