Are his-tagged proteins active???

Kasper Engkilde kasper at kemi.aau.dk
Thu Oct 10 08:47:16 EST 1996


Thank you for you interest...

I'm considering starting on a project where I will put a His-tag on my
protein(s) but are conserned that I can't trust it to be active. My
question is therefore: 

What experience do you have with the activity of his-tagged proteins???

Good and bad are of cause equally welcome!

On before hand - Thank you for your time and help.

Sincerely Kasper Dreyer Engkilde



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