Deborah_Britt at brown.edu (Deb Britt) wrote:
>What you need is a "Pap pen", which you use by simply drawing a circle
>around your tissue on the slide. The pen leaves behind a hydrophobic
>barrier that keeps your antibody solution neatly in place, over your
>tissue. We get ours from Research Products International (in the US
another possibility is to use a diamond pen to mark a ring around the
section. Worked quite well for me. Do all incubations in a wet chamber
(petri dish with the bottom covered with wet tissues).