How do I incubate slide-mounted sections with Ab?

Dr E. Buxbaum EB15 at le.ac.uk
Mon Oct 21 08:05:36 EST 1996


Deborah_Britt at brown.edu (Deb Britt) wrote:
>Ron-
>What you need is a "Pap pen", which you use by simply drawing a circle
>around your tissue on the slide.  The pen leaves behind a hydrophobic
>barrier that keeps your antibody solution neatly in place, over your
>tissue.  We get ours from Research Products International (in the US
>1-800-323-9814).

another possibility is to use a diamond pen to mark a ring around the 
section. Worked quite well for me. Do all incubations in a wet chamber 
(petri dish with the bottom covered with wet tissues). 




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