In article <53ilh3$3k5 at news.uni-c.dk>,
peider at biobase.dk (jacob hofman-bang) wrote:
>I AM GOING TO USE T3 AND T7 PRIMERS IN THE BLUESCRIPT PLASMID SYSTEM
>>CAN ANY OF YOU HELP ME WITH Tm, AMOUNT OF Mg2+ ETC..
>
I use an annealing temp of 60 degrees with a [MgCl2] of 2.5 mM. Good
luck.
Paul "Goose" Ulanch
Dept. Biochem/Biophys
Texas A&M University
College Station, TX 77843-2128
(409) 845-0832
PULANCH at bioch.tamu.edu
