PCR CONDITIONS FOR T3 AND T7 PRIMERS

Paul Ulanch Pulanch at bioch.tamu.edu
Mon Oct 21 10:51:32 EST 1996


In article <53ilh3$3k5 at news.uni-c.dk>,
   peider at biobase.dk (jacob hofman-bang) wrote:
>I AM GOING TO USE T3 AND T7 PRIMERS IN THE BLUESCRIPT PLASMID SYSTEM 
>
>CAN ANY OF YOU HELP ME WITH Tm, AMOUNT OF Mg2+ ETC..
>


I use an annealing temp of 60 degrees with a [MgCl2] of 2.5 mM.  Good 
luck.

Paul "Goose" Ulanch

Dept. Biochem/Biophys
Texas A&M University
College Station, TX   77843-2128
(409) 845-0832
PULANCH at bioch.tamu.edu



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