Selective PCR amplification

Doug Johnson djohnson at OREO.CHEM.UOTTAWA.CA
Mon Oct 21 09:51:20 EST 1996


We are trying to clone several closely related sequences via PCR 
using the same primers. The products are different sizes, from 300 to 
600 bp. When we amplify and clone, we have to screen many recombinant 
before we can find the smaller ones. Is it possible to control the PCR 
conditions e.g., by lowering the time of extension, in order to selectively 
amplify the smaller products?

Any suggestions greatfully considered.

Doug Johnson
Dr. D.A. Johnson
Plant Molecular Biology
Department of Biology
University of Ottawa
30 Marie Curie
Ottawa, Ontario
K1N 6N5
1-613-562-5800 ext 6347
1-613-562-5486(FAX)



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