How does degraded DNA look on gel?
Magdalena Cano Plewinska
mplewinska at earthlink.net
Mon Oct 21 16:29:37 EST 1996
Newswatcher at sickkids.on.ca (Newswatcher) wrote:
>Is it just a smear? Or will I see couple of bands also?
>2 of my DNA samples showed same bands on top of slight smearing upon
>electrophoresis, though most of the DNA are above 10kb. Just wondering if
>that's degradations or contaminations.
Could the smear you are seeing be RNA? Did you RNAse your prep before
putting it on the gel?
- Magdalena M. Plewinska, MD
University of Miami, Miami, FL, USA
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