Factors affecting the effciency of end-labelling by DNA kinase

:-Peter obrien at pharm.med.upenn.edu
Mon Oct 21 13:24:47 EST 1996


In article <54g99r$tn5 at enyo.uwa.edu.au>, dvoon at cyllene.uwa.edu.au (Dominic
Voon) wrote:

@--> Hi all,
@-->         I am going to start labelling some PCR products using DNA kinase
@--> (with alpha-32P dCTP) and it is quite important that I get high
@--> effeciency. I am just wondering if anyone could just give me a quick
@--> run-down on the critical factors that contribute to the effeciency of this
@--> experiment ? such as DNA concentration, dCTP concentration etc. Any
@--> pointer/comment/suggestion/advice will be much appreciated. Please email
@--> me directly at dvoon at cyllene.uwa.edu.au.
@--> 
@--> many thanks in advance,
@--> dominic


Make sure there are no ammonium ions in your solutions!

:-Peter  








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