I have been trying to produce a 35-S labelled protein using Promega's
rabbit reticulocyte lysate kit and am having problems as one of the
fragments of the active enzyme is approximately 10kD and it is being
masked by Haeme proteins which run at about the same position. Has
anyone any suggestions on removing the haeme protein or know of a method
of preventing it picking up the radiolabel?
Thanks in advance.
MRC Toxicology Unit,
University of Leicester.
DLR2 at le.ac.uk