In vitro translation problems

D.L. Roberts DLR2 at le.ac.uk
Tue Oct 22 06:16:01 EST 1996


I have been trying to produce a 35-S labelled protein using Promega's 
rabbit reticulocyte lysate kit and am having problems as one of the 
fragments of the active enzyme is approximately 10kD and it is being 
masked by Haeme proteins which run at about the same position.  Has 
anyone any suggestions on removing the haeme protein or know of a method 
of preventing it picking up the radiolabel?

Thanks in advance.

Darren Roberts 

MRC Toxicology Unit,
University of Leicester.

DLR2 at le.ac.uk




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