Storage of JM105/JM109

Greg Davis gdavis at
Mon Oct 21 19:44:07 EST 1996

Recently we have had problems with E. coli JM105 and JM109 dying at -20
C in 15% glycerol/LB.  We have never had this problem with NM522 or
DH5alpha at -20 C.

Are JM105 and JM109 strains this temperature sensitive?

We store bacterial, protein, and DNA samples at both -20 C and -80 C. 
We keep working glycerol cultures in the -20 C fridge and use the -80
samples for emergency back up.

gdavis at
University of Oklahoma
Chemical Engineering and Materials Science

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