liebert at bscr.uga.edu wrote:
>> We are switching to fluorescence DNA labelling methods now that we have access
> to Molecular Dynamics Fluoroimager and Phosphorimager systems. I would like
> to know what direct fluorescence labelling kits/methods are currently being used
> and with what success. We perform Southern blots, slot blots, PCR primer, PCR
> product, and oligo labelling. Molecular Dynamics recommends Amersham's Vistra
> ECF random prime labelling and signal amplification kit, and Vistra ECF oligolabelling and signal amplification kit. Any
comments??
Hi!
There is a kit from Kreatech Diagnostics for labelling single-
and doublestranded DNA or RNA with digoxigenin, fluorescin or
biotin. It works with a so calls universal linkage system, that
is al platinum compound, over which the marker group is bound on
about 90 % of the G in your fragment.
I used the digoxigenin-version of the kit for labelling oligos
for in situ hybridization, but without good results. But perhaps
the fluorescin-variant is better...
Kreatech is a company in Amstedam, Netherlands.
That´s the number for asking, who is the Kreatech-distributor in
your area: Phone: +31 20 691 91 81
Fax: +31 20 696 35 31
Good luck Gabriele Kerber
