In article <54gf55$7p4 at hobbes.cc.uga.edu>, liebert at bscr.uga.edu wrote:
> We are switching to fluorescence DNA labelling methods now that we have access
> to Molecular Dynamics Fluoroimager and Phosphorimager systems. I would like
> to know what direct fluorescence labelling kits/methods are currently
> and with what success. We perform Southern blots, slot blots, PCR primer, PCR
> product, and oligo labelling. Molecular Dynamics recommends Amersham's
> ECF random prime labelling and signal amplification kit, and Vistra ECF
and signal amplification kit. Any comments??
Any biotin or DIG labelling kit is convertable to the use on Fluoroimager
if you use
Amersham's "Signal amplification module" reagents and a matching antibody-AP.
I don't know if Attophos is still available separately from JBL
Scientific, as it used to be.
Alexander Kraev, PhD
Biochemie III, ETHZ Zurich
e-mail kraev at bc.biol.ethz.ch