What happens when you crosslink nucleic acid to nylon?

Hiranya Roychowdhury hroychow at NMSU.EDU
Tue Oct 22 09:55:07 EST 1996


At 02:04 PM 10/21/96 -0700, Edmundo Castro wrote:
>When crosslinking with UV what/where are the covalent bonds made?  With which 
>part of the nucleic acid and nylon?
>
>Also, I have assumed that when you crosslink RNA to a positively charged
membrane 
>by using NaOH you deprotonate the 2'hydroxy, the anion then acts as a
nucleophile 
>and it attacks a positive center in the nylon - would that be accurate?
>
Correct me if I'm wrong, but wont NaOH destroy the RNA?


>Thank you to all in advance!
>
>E. Castro-Vargas
>





>
Dr. Hiranya Sankar Roychowdhury
Plant Genetic Engineering Lab.
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at nmsu.edu




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