At 02:04 PM 10/21/96 -0700, Edmundo Castro wrote:
>When crosslinking with UV what/where are the covalent bonds made? With which
>part of the nucleic acid and nylon?
>>Also, I have assumed that when you crosslink RNA to a positively charged
>by using NaOH you deprotonate the 2'hydroxy, the anion then acts as a
>and it attacks a positive center in the nylon - would that be accurate?
>Correct me if I'm wrong, but wont NaOH destroy the RNA?
>Thank you to all in advance!
>Dr. Hiranya Sankar Roychowdhury
Plant Genetic Engineering Lab.
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at nmsu.edu