Nylon membrane stripping: deionized formamide....

Florence CABON cabon at infobiogen.fr
Tue Oct 22 10:05:40 EST 1996


Colin Rasmussen wrote:
> 
> In article <326BE46A.4183 at physci.ucla.edu>, ecastro at physci.ucla.edu wrote:
> 
> > Hello all!
> >
> > I was wondering what people use in order to strip their RNA nylon
> membranes.  I
> > have used boiling 0.1% SDS or 50% formamide 6XSSPE to strip uncharged and
> > positively charged membranes before.  The second method seems to work
> better.  Has
> > anyone tried something with wich they are happy with?
> >
> > Also, I have been using deoinized formamide:  is this a requirement when
> you are just
> > trying to strip the blot?
> 
> I use 0.1xSSC, 0.1% SDS, and boil for 5 minutes or so.  Seems to work very
> well for what we have done.
> 
> Colin
> 
> --
> Colin Rasmussen
> Molecular Mechanisms in Growth Control Group
> University of Alberta

I use 0,1% SDS in water, boil for 3 min, and let the membrane cool down
in water (large excess of water)
It works nicely. (except if you use oligos as probe)
-- 
======================================================
Florence Cabon. CNRS Villejuif, France. cabon at infobiogen.fr
======================================================



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