Nylon membrane stripping: deionized formamide....
cabon at infobiogen.fr
Tue Oct 22 10:05:40 EST 1996
Colin Rasmussen wrote:
> In article <326BE46A.4183 at physci.ucla.edu>, ecastro at physci.ucla.edu wrote:
> > Hello all!
> > I was wondering what people use in order to strip their RNA nylon
> membranes. I
> > have used boiling 0.1% SDS or 50% formamide 6XSSPE to strip uncharged and
> > positively charged membranes before. The second method seems to work
> better. Has
> > anyone tried something with wich they are happy with?
> > Also, I have been using deoinized formamide: is this a requirement when
> you are just
> > trying to strip the blot?
> I use 0.1xSSC, 0.1% SDS, and boil for 5 minutes or so. Seems to work very
> well for what we have done.
> Colin Rasmussen
> Molecular Mechanisms in Growth Control Group
> University of Alberta
I use 0,1% SDS in water, boil for 3 min, and let the membrane cool down
in water (large excess of water)
It works nicely. (except if you use oligos as probe)
Florence Cabon. CNRS Villejuif, France. cabon at infobiogen.fr
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