Amy Boedeker wrote:
>> Hello,
>> I am working on the Hageman Factor gene in mice and cats. I have
> recently been trying to retrieve a correct 5' gene sequence (exons
> 1-3) from a cat cDNA library (gt11, made by Clonetech using cat liver
> in 1991). I have retrieved the rest of the cDNA successfully (exons
> 4-14).
><snipped>
If RNA supply is no problem, try 5' RACE. We have good luck with the
kit from Life Technologies (Gibco/BRL).
AJW
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John Watson
Bristol-Myers Squibb Co.
watson_j at bms.com
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"If you're not part of the solution, you're part of the precipitate."
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