Finding 5' sequence of cDNA

John Watson watson_j at bms.com
Tue Oct 22 17:14:03 EST 1996


Amy Boedeker wrote:
> 
> Hello,
> 
> I am working on the Hageman Factor gene in mice and cats.  I have
> recently been trying to retrieve a correct 5' gene sequence (exons
> 1-3) from a cat cDNA library (gt11, made by Clonetech using cat liver
> in 1991).  I have retrieved the rest of the cDNA successfully (exons
> 4-14).
> 
<snipped>

If RNA supply is no problem, try 5' RACE.  We have good luck with the 
kit from Life Technologies (Gibco/BRL).

AJW
----------------
John Watson
Bristol-Myers Squibb Co.
watson_j at bms.com
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