Yi Zhang (BIO) wrote:
>> Hi there:
>> I want to find a protocol for pulse field eletrophoresis to isolate a
> large piece of DNA fragment. Does anyone have a protocol for pulse field
> electrophoresis which works well in your hands to separate DNA freagment?
The answer to this question depends on whether you are looking for a
switching protocol to resolve the fragment (if so how large is the
fragment) or a protocol for recovering it from the gel. Additional
information would help in trying to answer your question.
Dr. Richard Goering
Dept. Med. Microbiol. and Immunol.
Creighton Univ. Sch. Med.