Hello!
I have a problem in purification of an insect virus.
When viral particles were collected (as a white band) in sucrose
gradients (30%/60%), I transferred the band to a new ultracentrifuge
tube.
Then, I spun the tube at 100,000g for 1 hr. But, I have no get any
pellet. I thought that viruses have no spun down because of
sucrose buoyancy.
So, I allowed the solution at 4oC, overnight, stirred it roughly and
ultracentrifuge at 100,000g for 1 hr. But, I have no any pellet, too!
Is there anyone know about it?
Why virus particles were spun down?
What can I do for purification of the virus particles?
Your Sincerely, Min-Ho Lee
--
mhlee at bric.postech.ac.kr
Web for the Korean Entomologist (my home page):
http://bric.postech.ac.kr/about/mhlee/
BRIC (Biological Research Information Center), POSTECH
http://bric.postech.ac.kr
--
