Need help with mRNA turnover using pulse-chase

Taru Kosonen ez007012 at chip.ucdavis.edu
Tue Oct 22 18:13:57 EST 1996


I'm trying to determine the halflife of some relatively low abundant
transcrpits in human skin fibroblasts using the pulse-chase method with
3-H -uridine. Anybody familiar with this method? My specific concern is
about how to take the reincorporation/recycling of the label into account?

 Any help/hints are greatly appreciated. You can also answer directly by
email: takosonen at ucdavis.edu

Thanks. Taru Kosonen
	Dept. of Nutrition
	UC DAVIS



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