I'm trying to determine the halflife of some relatively low abundant
transcrpits in human skin fibroblasts using the pulse-chase method with
3-H -uridine. Anybody familiar with this method? My specific concern is
about how to take the reincorporation/recycling of the label into account?
Any help/hints are greatly appreciated. You can also answer directly by
email: takosonen at ucdavis.edu
Thanks. Taru Kosonen
Dept. of Nutrition