Greg Davis <gdavis at uoknor.edu> wrote:
>Recently we have had problems with E. coli JM105 and JM109 dying at -20
>C in 15% glycerol/LB. We have never had this problem with NM522 or
>DH5alpha at -20 C.
>Are JM105 and JM109 strains this temperature sensitive?
>We store bacterial, protein, and DNA samples at both -20 C and -80 C.
>We keep working glycerol cultures in the -20 C fridge and use the -80
>samples for emergency back up.
>gdavis at ou.edu>University of Oklahoma
>Chemical Engineering and Materials Science
We store all bacteria and transformed bacteria in 50% glycerol at
-80 C . Using storage in this manner we have never had a problem with
stocks dying off.
SWB at Di1.evms.edu
Molecular Biology Laboratory
The Diabetes Research Institutes
Eastern Virginia Medical School