Storage of JM105/JM109

[Rover]

Greg Davis <gdavis at uoknor.edu> wrote:

>Recently we have had problems with E. coli JM105 and JM109 dying at -20
>C in 15% glycerol/LB.  We have never had this problem with NM522 or
>DH5alpha at -20 C.

>Are JM105 and JM109 strains this temperature sensitive?

>We store bacterial, protein, and DNA samples at both -20 C and -80 C. 
>We keep working glycerol cultures in the -20 C fridge and use the -80
>samples for emergency back up.

>Greg
>gdavis at ou.edu
>University of Oklahoma
>Chemical Engineering and Materials Science

We store all bacteria and transformed bacteria in 50% glycerol at 
-80  C . Using storage in this manner we have never had a problem with
stocks dying off.



SWB at Di1.evms.edu
Molecular Biology Laboratory
The Diabetes Research Institutes
Eastern Virginia Medical School