Are you using T4 kinase to perform end-labelling? I would use
gamma-32P-ATP. Otherwise, use DNA polymerase for random labelling.
Johns Hopkins University
Molecular Diagnostic Lab.
On 21 Oct 1996, Dominic Voon wrote:
> Hi all,
> I am going to start labelling some PCR products using DNA kinase
> (with alpha-32P dCTP) and it is quite important that I get high
> effeciency. I am just wondering if anyone could just give me a quick
> run-down on the critical factors that contribute to the effeciency of this
> experiment ? such as DNA concentration, dCTP concentration etc. Any
> pointer/comment/suggestion/advice will be much appreciated. Please email
> me directly at dvoon at cyllene.uwa.edu.au.>> many thanks in advance,
> Dominic Voon .***. .***. .***. .***.
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> Email:dvoon at cyllene.uwa.edu.auhttp://www.uwa.edu.au/cyllene/dvoon> Check out the OCF Perth Home Page: