Factors affecting the effciency of end-labelling by DNA kinase

XIAO-MIN SU sumini at welchlink.welch.jhu.edu
Tue Oct 22 16:54:57 EST 1996


Hi,

Are you using T4 kinase to perform end-labelling? I would use 
gamma-32P-ATP. Otherwise, use DNA polymerase for random labelling.

Xiao-min Su
Johns Hopkins University
Molecular Diagnostic Lab.

On 21 Oct 1996, Dominic Voon wrote:

> Hi all,
> 	I am going to start labelling some PCR products using DNA kinase
> (with alpha-32P dCTP) and it is quite important that I get high
> effeciency. I am just wondering if anyone could just give me a quick
> run-down on the critical factors that contribute to the effeciency of this
> experiment ? such as DNA concentration, dCTP concentration etc. Any
> pointer/comment/suggestion/advice will be much appreciated. Please email
> me directly at dvoon at cyllene.uwa.edu.au.
> 
> many thanks in advance,
> dominic
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