Factors affecting the effciency of end-labelling by DNA kinase
sumini at welchlink.welch.jhu.edu
Tue Oct 22 16:54:57 EST 1996
Are you using T4 kinase to perform end-labelling? I would use
gamma-32P-ATP. Otherwise, use DNA polymerase for random labelling.
Johns Hopkins University
Molecular Diagnostic Lab.
On 21 Oct 1996, Dominic Voon wrote:
> Hi all,
> I am going to start labelling some PCR products using DNA kinase
> (with alpha-32P dCTP) and it is quite important that I get high
> effeciency. I am just wondering if anyone could just give me a quick
> run-down on the critical factors that contribute to the effeciency of this
> experiment ? such as DNA concentration, dCTP concentration etc. Any
> pointer/comment/suggestion/advice will be much appreciated. Please email
> me directly at dvoon at cyllene.uwa.edu.au.
> many thanks in advance,
> Dominic Voon .***. .***. .***. .***.
> Department of Biochemistry *| | | * | | | * * | | | * | | | *
> University of Western Australia | | * * | | | * * | | | * * | | | *
> Nedlands 6907,Perth * | | * * | | | * | | | * * | | | *
> Australia '***' '***' '***' '***'
> Tel: (609) 380 2303 (w) Fax: (609) 380 1148
> 386 6036 (h)
> Email:dvoon at cyllene.uwa.edu.au http://www.uwa.edu.au/cyllene/dvoon
> Check out the OCF Perth Home Page:
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