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PCR primers for common plasmids

Olivier Gandrillon ogandril at cri.ens-lyon.fr
Wed Oct 23 03:24:41 EST 1996


I sometimes have been experiencing amplification trouble with standard  
primers when used either in PCR or sequencing (using Taq). Therefore I 
have devised new, longer primers,  with higher Tm. My primers are to be 
used on pBSK. I can post their sequence if someone is interested.


Ethan Strauss wrote:
> Hi everyone,
>     I have been getting clones from the Arabidopsis EST bank. I would
> like to be able to amplify the inserts using primers directed against
> the vector. Most of these clones are in lambda ZipLox which has T7 and
> SP6 RNA polymerase transcription sites and M13 forward and reverse
> sites.
>      Can anyone give me advice on primer design for PCR using these
> sites? Will the standard sequencing primers sequences work?
> If I do go with sequencing primers is it always cheaper to custom order
> them to buy them as sequencing primers? Why?
> Thanks for your help,
> Dr. Ethan Strauss
> 310 Madigan
> University of Illinois
> Urbana IL, 61801
> 217-244-3230
> eestraus at staff.uiuc.edu

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