EST-insert size

[Zhonglin Chai]

Dear Fabian,

I like to know the answer too. I guess that they might sequence any clone one 
round, and deposited the data in the database. If you compared your sequence 
with a matched EST sequence, you would find there were more and more mismatchs 
at the 3' end, indicating that the EST sequences could be determined once by 
one priming reaction.

> To: methods at net.bio.net
> 
> From: "FABIAN MOEBIUS" <Fabian.Moebius at uibk.ac.at>
> 
> Subject: EST-insert size
> 
> Date: 23 Oct 1996 19:39:21 +0100
> 
> Discrepancy actual EST-insert size and length of the database
> sequence
> 
> Dear collegues,
> I recently posted a message that EST clones obtained from a Resource
> centre did not contain any insert. Somebody suggested to order clones
> from an another centre which worked nice because these clones
> have inserts. But only one from five different ESTs carries an insert
> of the
> expected size (0.5 kb) wheras the other inserts are larger (around
> one kb). I regret if the question is stupid, but do the EST people
> only enter
> the size of the SEQUENCED DNA into the database and ignore the rest
> of the clone.
> If so why do they not give the insert size?
> If not should I dump the 1 kb clones or are they worth further
> characterization and sequencing.
> Thanks
> Dr. Fabian F. MOEBIUS
> Institut fuer Biochemische Pharmakologie
> Peter Mayr Str. 1
> 6020 Innsbruck
> AUSTRIA
> TEL ++43-512-507-3155
> FAX ++43-512-588627
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