In article <326D48D0.796D at bms.com>, watson_j at bms.com wrote:
> Ed wrote:
>> > I had a little question...I have screened a cDNA library made in Lambda
> > ZAPII, which has self excision of pBluescript when the phage is plated with
> > a certain helper phage (which we conveniently do NOT have >:( ). Now, my
> > question is...since budgetary constraints are a bit of a problem (and
> > getting the kit for excision is a huge waste, since I'll be using it once !)
> > should there not be a way of cutting out the pBluescript out of the
> > lambda phage, and re-circularizing it ? I mean...from the vector map, it
>> A quick glance at the Stratagene catalog informs me that the ExAssist
phage (which
> I've used with excellent results) are $95.00. Not much when you
consider the price
> of many restriction enzymes. And the phage are stable for years in the
freezer
> (the tube I used was over 2 years old and had been purchased by an earlier
> post-doc), so who's to say you'll only be using it once?
You can also use either R408 or M13K07 phage in XL-1 blue cells too. They
are usually pretty easy to come by.
Colin
--
Colin Rasmussen
Molecular Mechanisms in Growth Control Group
University of Alberta
