IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

lambda ZAPII clones...

Colin Rasmussen colin at fungus.biochem.ualberta.ca
Wed Oct 23 11:09:55 EST 1996

In article <326D48D0.796D at bms.com>, watson_j at bms.com wrote:

> Ed wrote:
> > I had a little question...I have screened a cDNA library made in Lambda
> > ZAPII, which has self excision of pBluescript when the phage is plated with
> > a certain helper phage (which we conveniently do NOT have >:( ). Now, my
> > question is...since budgetary constraints are a bit of a problem (and
> > getting the kit for excision is a huge waste, since I'll be using it once !)
> > should there not be a way of cutting out the pBluescript out of the
> > lambda phage, and re-circularizing it ?  I mean...from the vector map, it
> A quick glance at the Stratagene catalog informs me that the ExAssist
phage (which 
> I've used with excellent results) are $95.00.  Not much when you
consider the price 
> of many restriction enzymes.  And the phage are stable for years in the
> (the tube I used was over 2 years old and had been purchased by an earlier 
> post-doc), so who's to say you'll only be using it once?

You can also use either R408 or M13K07 phage in XL-1 blue cells too.  They
are usually pretty easy to come by.


Colin Rasmussen
Molecular Mechanisms in Growth Control Group
University of Alberta

More information about the Methods mailing list

Send comments to us at biosci-help [At] net.bio.net