help: virus purification problem

brett brett at BORCIM.WUSTL.EDU
Wed Oct 23 11:07:21 EST 1996


>Hello!
>I have a problem in purification of an insect virus. 
>When viral particles were collected (as a white band) in sucrose 
>gradients (30%/60%), I transferred the band to a new ultracentrifuge
>tube.
>Then, I spun the tube at 100,000g for 1 hr. But, I have no get any 
>pellet. I thought that viruses have no spun down because of 
>sucrose buoyancy. 
>So, I allowed the solution at 4oC, overnight, stirred it roughly and
>ultracentrifuge at 100,000g for 1 hr. But, I have no any pellet, too!
>
>Is there anyone know about it?
>Why virus particles were spun down?
>What can I do for purification of the virus particles?
>
>Your Sincerely, Min-Ho Lee
>--
>mhlee at bric.postech.ac.kr
>Web for the Korean Entomologist (my home page):
>http://bric.postech.ac.kr/about/mhlee/
>BRIC (Biological Research Information Center), POSTECH
>http://bric.postech.ac.kr
>--

Well, you could dialyze out the sucrose, but I doubt this is the problem if
you dilute it into homogeneity. You didn't mention the virus or the number
of particles in your prep, but it's possible the virus pellet was simply
too small to see. 


Brett Lindenbach
    
Program in Immunology                              
Washington University - St Louis                  
brett at borcim.wustl.edu                             

"I own my own pet virus. I get to pet and name her." - Cobain




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