ligation of a 8kb fragment

[Corinne BELVILLE]

I would like to realize a ligation of a fragment whom the size is 8kb. My
ligation failed in vector pGEM-T (at 15°C).I put 5 or 20 more inserts than
plasmid.
Is it necessary to change of vector?  is it better to work at +4°C?

Thank you for your answers


Corinne Belville

-- 
Daniele EUSEBE(Ecole Normale Supérieure-INSERM U293-Paris-France)
e-mail:deusebe at wotan.ens.fr