Are his-tagged proteins active???

[Steven Goldberg]

In article <325CFE64.7D30 at kemi.aau.dk>, Kasper Engkilde
<kasper at kemi.aau.dk> wrote:

> Thank you for you interest...
> 
> I'm considering starting on a project where I will put a His-tag on my
> protein(s) but are conserned that I can't trust it to be active. My
> question is therefore: 
> 
> What experience do you have with the activity of his-tagged proteins???
> 
> Good and bad are of cause equally welcome!
> 
> On before hand - Thank you for your time and help.
> 
> Sincerely Kasper Dreyer Engkilde