DNA onto metal

P. Jones BGYPJ at leeds.ac.uk
Thu Oct 24 05:33:27 EST 1996


In article <podar-1810962001390001 at 129.112.42.147>,
   podar at utsw.swmed.edu (Mircea Podar) wrote:
>In article <54627h$h04 at news3.realtime.net>, Lesley Lakey
><LLakey at CarboMedics.com> wrote:
>
>> I am looking for methods of precipitating plasmid DNA onto metals such as 
>> gold, platinum, and iridium.  Any suggestions?  I am doing gene therapy 
>> research and need a quick release of DNA to the area of interest.
>>                                                  
>>                                                   Thanks,
>>                                                   L. Lakey
>
>
>
>Check the article published in Methods in Enzymology, vol 264 (1996),
>pages 265-278. That describes a protocol for coating gold microbeads with
>plasmid DNA for use in the mitochondrial transformation of yeast with the
>"biolistic gun". It works also for tungsten. 
>
>Mircea

I agree but if using tungsten make sure its fresh and not oxidised.

Paul Jones



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