-cycle sequencing problem

z un691cs at genius.embnet.dkfz-heidelberg.de
Thu Oct 24 03:35:13 EST 1996


Hello,
we are cycle sequencing with Thermosequenase from amersham,
and there is one stretch that is impossible to read with any 
primer pair that we use. we do not know whether it is just CCG 
or CCGG or CCCGG. the sequence surrounding this problemspot
is not very GC rich.
Any suggestions ? can one use DMSO, and at what concentration ?

z




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