oligos vs others

Chihara chihara at usfca.edu
Thu Oct 24 16:56:08 EST 1996


John Watson wrote:
> 
> Barney P. Crum wrote:
> >
> > I'm currently doing northerns using a 35mer oligo to probe for a specific
> > RNA sequence.  I am having trouble getting the necessary signal generated
> > and was wondering if other probes may work better for Northern hybridization
> > (i.e. a larger probe or RNA probe).  I know that the mRNA is in high enough
> > abundance to detect it using in situ hybridization.  Any ideas will greatly
> > help.  Thanks.
> 
> The only time I probed a Northern with an oligo, I used a 43mer.  Not *much*
> different from your probe.  It worked quite nicely, though the exposure time was
> kinda long.  I calculated that the specific activity was 4x10E8/ug (I quoting
> from the paper - no notes handy).  I'll send you my oligo hyb protocol if you
> wish - it's ridiculously easy.
> 
I would like very much to see your protocol.  I have an undergraduate 
about to try a Northern with a 21mer and I have never done it.  Any 
help for comparison's sake is appreciated.

Your mailing address returned so I am posting in hope you will send it 
to me too, or post here.
thanks



More information about the Methods mailing list