oligos vs others
chihara at usfca.edu
Thu Oct 24 16:56:08 EST 1996
John Watson wrote:
> Barney P. Crum wrote:
> > I'm currently doing northerns using a 35mer oligo to probe for a specific
> > RNA sequence. I am having trouble getting the necessary signal generated
> > and was wondering if other probes may work better for Northern hybridization
> > (i.e. a larger probe or RNA probe). I know that the mRNA is in high enough
> > abundance to detect it using in situ hybridization. Any ideas will greatly
> > help. Thanks.
> The only time I probed a Northern with an oligo, I used a 43mer. Not *much*
> different from your probe. It worked quite nicely, though the exposure time was
> kinda long. I calculated that the specific activity was 4x10E8/ug (I quoting
> from the paper - no notes handy). I'll send you my oligo hyb protocol if you
> wish - it's ridiculously easy.
I would like very much to see your protocol. I have an undergraduate
about to try a Northern with a 21mer and I have never done it. Any
help for comparison's sake is appreciated.
Your mailing address returned so I am posting in hope you will send it
to me too, or post here.
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